GI Map Test UK with Zonulin

 

The GI-MAP Test UK is a comprehensive DNA-based stool test that analyses bacteria, parasites, viruses, fungi/yeast, digestion markers, inflammation, and zonulin (a marker linked to intestinal permeability or “leaky gut”).

It uses advanced qPCR (quantitative polymerase chain reaction) technology to detect even low levels of microbial DNA that standard NHS stool tests do not measure.

Our GI-MAP test is analysed by  Diagnostic Solutions and includes zonulin as standard.  The test costs £345, and is available across the UK via a home testing kit or at our London clinic.

Key Facts GI-MAP Test UK

– Test type: Stool PCR DNA test (qPCR)

– Includes: Zonulin (intestinal permeability marker)

– Cost: £345 (GBP)

– Availability: UK-wide home test or London clinic

– Laboratory: Diagnostic Solutions (USA)

– Sample required: 1 stool sample

– Turnaround time: Approximately 3 weeks

 

What is the difference between a GI-MAP and a NHS stool test?

 

Most stool tests available through the NHS are designed to detect  inflammation and rule out Crohn’s or Ulcerative Colitis as well as  Salmonella, E. coli, Shigella or Campylobacter.

These tests are very useful but they do not provide information about other pathogens.

The GI MAP looks at a number of markers that are not covered on the NHS including yeast, opportunistic bacteria, parasitic infections, digestive and absorption or Zonulin- a marker linked to ‘leaky gut’.

The GI MAP test may help with the following conditions:

  • IBS- Irritable bowel Syndrome
  • Skin conditions like acne and psoriasis
  • Mood disorders such as depression and anxiety
  • Autoimmune conditions
  • Fatigue and brain fog
  • Weight management challenges
  • Diarrhea and digestive problems
  • Foodborne illnesses
  • Gastritis and viral gastroenteritis
  • Gut dysbiosis (microbial imbalance)
  • Inflammation and immune-related gut issues
  • Detection of bacterial, parasitic, or viral infections (including antibiotic-resistant strains)
  • Leaky gut

The test is carried out by Diagnostic Solutions.

How much does GI-MAP test cost UK?

Our GI Map test  includes Zonulin and the fee £345. We offer the test at our clinic in London or via post at home.

GI-MAP test at Home

Most pateints carry out the GI Map test  at home. It is easy,  we can post you a kit, you then provide a stool sample and send it back to the laboratory for analysis.

What Does the GI-MAP test measure?

 

The GI-MAP stool test analyses a wide range of microorganisms and functional markers that influence digestion, immunity, inflammation, and gut barrier integrity.

Please see a GI-MAP stool test UK sample report here:

 

It provides insight into:

– Beneficial, opportunistic, and pathogenic bacteria
– Viruses
– Fungi and yeast (including Candida species)
– Parasites and worms
– Markers of inflammation and immune activity
– Digestive function and absorption
– Antibiotic resistance genes
– Zonulin (a marker associated with intestinal permeability or “leaky gut”)

 

The test screens for organisms and imbalances that may contribute to gastrointestinal symptoms such as bloating, diarrhoea, constipation, IBS-like symptoms, recurrent infections, and food reactions.

Key categories Measured on the GI-MAP test

  • **Zonulin**
    Included as standard
    – A marker associated with intestinal permeability (“leaky gut”)

  • *Beneficial bacteria**

    – Includes species such as *Bacteroides fragilis*, *Bifidobacterium spp.*, *Lactobacillus spp.*, *Akkermansia muciniphila*, *Faecalibacterium prausnitzii*, *Enterococcus spp.*, and *Roseburia spp.*

  • **Opportunistic / dysbiotic bacteria**

    – Includes organisms such as *Klebsiella*, *Enterobacter cloacae*, and *Streptococcus*
    – These may be associated with bloating, diarrhoea, IBS, or SIBO-like symptoms when overgrown
  • **Pathogenic bacteria**

– Includes species such as *Clostridioides difficile*, *Salmonella*, and *E. coli O157*
– These are commonly linked to food poisoning and acute diarrhoeal illness

**Yeast and fungal organisms**
– Includes *Candida albicans*, *Geotrichum*, *Microsporidium*, and *Rhodotorula*
– Overgrowth may be associated with bloating, sugar cravings, fatigue, and recurrent thrush

**Parasites**
– Includes organisms such as *Blastocystis hominis* and *Giardia*
– These may contribute to diarrhoea, abdominal pain, and nutrient malabsorption

 

 

 

What makes the GI-MAP test different?

 Instead of a vague “present/absent,” GI-MAP measures how much, so you and your practitioner can see what’s driving bloating, IBS symptoms, skin flares, fatigue, or persistent gut issues and treat them with confidence.

  • Quantified results, not guesses – The test is able to measures the load of key organisms (bacteria, parasites, and yeast such as Candida), plus beneficial species and dysbiosis patterns.

  • It includes gut-inflammation and digestion markers (e.g., calprotectin, secretory IgA, pancreatic elastase, beta-glucuronidase, and fecal fat) to assess barrier integrity, immune tone, and digestive efficiency.

  • Pathogens – Screens for bacteria, yeast, viruses  and H. pylori (with reported virulence factors)

Who should use the test? If you have IBS/IBD-like symptoms, reflux, bloating, constipation or diarrhoea, unexplained fatigue or brain-fog, skin concerns (acne/rosacea/eczema), recurrent infections, or stubborn symptoms after antibiotics, travel, or food poisoning, this test may be very helpful.

How can I interpret results?

We offer consultations at our London Harley Street clinic or via Zoom to help detailed interpretation of the test results and will recommend supplements, and dietary advice.

Case Study- James

James had long-standing IBS-C and post-meal bloating; His GI-MAP test showed an overgrowth of yeast and mild dysbiosis. After a 4-week plan (diet, anti-fungals and probiotics) his stools became more regular and bloating reduced.

 

Case Study – Kate

Kate had cyclical acne with bloating/food reactions; The GI-MAP showed Candida overgrowth and elevated β-glucuronidase. A 8-week plan (phased low-sugar diet, microbiome-supportive fibre, targeted nutraceuticals) led to calmer skin and less bloating by weeks 6–8.

 

What Parasites does the GI-Map test Detect?

 

The GI-MAP test UK identifies a variety of  parasites and pathogens.

It covers protozoa (Giardia, Cryptosporidium) and helminths (worms) that may cause diarrhoea, nutrient deficiencies, or IBS-like symptoms.

Pathogens and Analytes
Parasites and Worms Detected by GI-MAP:

Parasites:
  • Blastocystis hominis
  • Giardia lamblia
  • Entamoeba histolytica
  • Cryptosporidium species
  • Dientamoeba fragilis
Protozoa:
  • Chilomastix mesnili
  • Cyclospora species
  • Endolimax nana
  • Entamoeba coli
  • Pentatrichomonas hominis
Bacterial Phyla:
  • Bacteroidetes
  • Firmicutes
  • Firmicutes/Bacteroidetes Ratio
Dysbiotic/Overgrowth Bacteria:
  • Bacillus species
  • Enterococcus faecalis
  • Enterococcus faecium
  • Morganella species
  • Pseudomonas species
  • Pseudomonas aeruginosa
  • Staphylococcus species
  • Staphylococcus aureus
  • Streptococcus species
  • Methanobacteriaceae (family)
Worms:
  • Ascaris lumbricoides (Roundworm)
  • Strongyloides stercoralis (Threadworm)
  • Ancylostoma duodenale and Necator americanus (Hookworm)
  • Trichuris trichiura (Whipworm)
Bacterial Pathogens:
  • Campylobacter
  • Clostridium difficile (Toxins A & B)
  • Enterohemorrhagic E. coli
  • E. coli O157
  • Enteroinvasive E. coli / Shigella
  • Enterotoxigenic E. coli (LT/ST)
  • Shiga-like Toxin E. coli (stx1, stx2)
  • Salmonella
  • Vibrio cholerae
  • Yersinia enterocolitica
  • Helicobacter pylori
    • Virulence factors: babA, cagA, dupA, iceA, oipA, vacA, virB, virD
Viral Pathogens:
  • Adenovirus 40/41
  • Norovirus GI
  • Norovirus GII
Potential Autoimmune Triggers:
  • Citrobacter species
  • Citrobacter freundii
  • Fusobacterium species
  • Klebsiella species
  • Klebsiella pneumoniae
  • Mycobacterium avium subsp. paratuberculosis
  • Prevotella species
  • Proteus species
  • Proteus mirabilis
Fungi / Yeast:
  • Candida albicans
  • Candida species
  • Geotrichum species
  • Microsporidia species
  • Rhodotorula species
Opportunistic Viruses:
  • Cytomegalovirus (CMV)
  • Epstein-Barr Virus (EBV)
Intestinal Health Markers:
Digestion:
  • Elastase-1
  • Steatocrit
Immune Response:
  • Secretory IgA (SIgA)-gut immune defence
  • Anti-gliadin SIgA- can be linked to gluten sensitivity
Inflammation:
  • Calprotectin – intestinal inflammation, also used in NHS IBD tests
Gastrointestinal Markers:
  • ß-Glucuronidase
  • Occult Blood (FIT)
Optional Antibiotic Resistance Genes (Phenotypes for Helicobacter):
  • Amoxicillin
  • Clarithromycin
  • Fluoroquinolones
  • Tetracycline
Methodology
  • Quantitative Polymerase Chain Reaction (qPCR): This method amplifies and quantifies microbial DNA to identify a wide range of pathogens and markers in the gut.
How accurate is the GI-Map test?

The GI-MAP uses quantitative polymerase chain reaction (qPCR), a highly sensitive and specific method for detecting microbial DNA. This molecular technology amplifies even small amounts of DNA, making it more accurate than traditional culture-based tests, which often miss certain microbes, especially those that can’t grow outside the body.

  • Sensitivity: qPCR can detect microorganisms at very low levels, meaning it can identify even small or hidden infections.
  • Specificity: The test is highly specific because it targets the genetic material of microbes, reducing the chances of false positives.

The GI-MAP test is capable of detecting strict anaerobes, viruses, and virulence factors—microbes and pathogens that often go undetected in traditional stool tests. This expands its ability to give a complete picture of gut health.

The test’s sensitivity can sometimes detect microbes at low levels that may not necessarily be causing illness or symptoms, which requires careful interpretation by a healthcare professional.

Shipping Instructions

 

To ensure accurate results, the GI MAP stool test must be completed within a single day, and the sample should be returned to the laboratory within 24 hours due to its brief stability period.

Collection Schedule:

  • Please plan to collect your  single stool sample on a Sunday or Monday or Tuesday.

Collection Timing:

  • You are free to collect your sample at any point during your designated collection day.
  • The results take 3 weeks.

 

 

 

What happens when my results are back?

We will email these to you within 3 weeks.

What are the Collection Instructions for the GI-MAP Stool Test?

 

  • The test only requires one stool sample.
  • Lactoferrin: If you’re taking lactoferrin, wait 72 hours after your last dose before collecting your sample.

  • Digestive aids & pain relief: Avoid digestive enzymes, antacids, and aspirin for 2 days before collection (unless your healthcare provider advises otherwise).

  • Antibiotics, antimicrobials & probiotics:

    • For antibiotics, antiparasitics, antifungals, probiotics, wait at least 14 days after your last dose before testing.

    • For antibiotics specifically, wait at least 14 days where possible (unless your doctor instructs otherwise).

  • Kit guide: Please read and follow all instructions included with your GI-MAP® stool test kit.

Never discontinue prescription medications without first consulting your doctor.

 

 

Can I book a consultation for Interpretation after receiving my GI MAP Test Results?

Yes, we do offer consultations in Harley street or via zoom.

  1. Review of Results:
    Your functional medicine practioner  will carefully review your results to identify any imbalances or pathogens that may be affecting your gut health.
  2. Understanding the Findings:
    The results will include information about various gut bacteria, viruses, parasites, and other markers that reflect your digestive health. We will explain what each finding means in relation to your symptoms and overall health.
  3. Personalized Treatment Plan:
    Based on your results, we will recommend a personalized treatment plan. This could include dietary changes, supplements, or other interventions designed to restore balance to your gut microbiome.
  4. Follow-Up:
    You may have a follow-up appointment to discuss your results in more detail, monitor your progress, and make any necessary adjustments to your treatment plan.
  5. Additional Testing:
    In some cases, we might suggest further testing if specific issues are identified that require more investigation.
  6. Lifestyle Recommendations:
    Alongside treatment options, we may offer lifestyle recommendations aimed at promoting a healthier gut, such as stress management techniques,  and exercise.

Frequently Asked Questions About the GI-MAP Test

1. What is the GI-MAP test?
The GI-MAP is a DNA-based stool test (qPCR technology) that analyses your gut microbiome, digestion, and immune markers. It can identify bacteria, parasites, viruses, and fungi, giving a much deeper picture of gut health than standard stool cultures.

2.GI-MAP Test vs NHS Stool Test – What’s the Difference? 
NHS stool tests are mainly designed to detect acute infections like Salmonella or E. coli. They don’t usually assess gut flora balance, yeast, or digestive function. GI-MAP, on the other hand, screens for a wide range of microbes and functional markers, helping to uncover the root causes of chronic gut symptoms.

3. What does the GI-MAP test measure?
The test looks at bacteria (both normal flora and opportunistic species), parasites, viruses, fungi/yeast, markers of digestion (like elastase-1), fat absorption (steatocrit), and inflammation/immune activity (calprotectin, secretory IgA). It also checks for Zonulin/leaky gut and antibiotic resistance genes.

4. Can I get the GI-MAP test on the NHS?
No, the GI-MAP test is not available on the NHS. It is only offered privately through registered practitioners and clinics.

5. How much does the GI-MAP test cost in the UK?
The test costs £345 and includes Zonulin a marker for leaky gut. The price includes  the kit, courier service, full lab report.

6. How do I collect a GI-MAP stool sample at home?
Only a small stool sample is needed. The sample is mixed with a stabilising solution, so it can be shipped safely at room temperature by courier.

7. How long does it take to get results?
Once the lab receives your sample, results are usually ready within 2–3 weeks. 

8. Do I need to stop supplements or medications before the test?
Yes, certain supplements and medications can affect results. For example, antibiotics, antifungals, and probiotics may need to be paused before testing. 

9. Is the GI-MAP test suitable for children?
Yes, the test can be used in children. However, treatment options may be different for children than for adults, so results should always be interpreted with a qualified practitioner.

10. Will the GI-MAP test help with IBS or other gut symptoms?
Yes. Many people with IBS, bloating, reflux, diarrhoea, or constipation have normal NHS stool results. The GI-MAP often reveals imbalances (such as elevated zonulin, yeast, parasites, or low digestive enzymes) that can guide a more targeted and personalised approach.

11. Can GI-MAP diagnose SIBO?
No—SIBO is diagnosed via breath testing. GI-MAP can reveal co-factors (e.g., dysbiosis, low elastase, H. pylori, inflammation) that sustain symptoms.

12. Does a positive organism always mean treatment?
Not necessarily—load values, symptoms and history guide decisions. Some findings are transient or clinically insignificant.

How long do GI-MAP test results take?

The turnaround time for results from the GI-MAP test is 2- 3 weeks. The results will be e-mailed to you.

Who should use the GI-Map test?

If you have symptoms of IBS and would like to test for leaky gut and inflammation as well as parasites, yeast and bacteria this test is a good choice. If you have upper gut symptoms you may wish to also consider a  SIBO test.

How can I order GI-MAP test?

Our GI-MAP testing can be sent to you at home – we ship all over the UK and Europe. If you would like to order a test you can order online call us on 0345 1297996 or Contact us

How does the test work?

Patients need to send in only one sample to the laboratory.

Have any questions? Call us now!

0345 1297996

FAQ

How is the GI-MAP test performed?

Patients need to send in one stool sample to the laboratory.

If you have any further questions or concerns, please feel free to contact our office.

Is Helicobacter covered on the GI-Map stool test?

Yes, Helicobacter is also covered on the stool test.

Can I get this test on the NHS?

Unfortunately, these tests are not currently offered on the NHS.

How are the stool samples preserved?

We will post you special containers that contain preservative liquid. You will need to put your stool specimens into these containers and courier them back to our lab for examination.

What happens if I need help with test?

We offer consultations at our sister clinic,  ibs-solutions.co.uk in Harley street and online by virtual appointments.